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Sample Test
Ch03
Multiple Choice Questions
|
1.
|
Eukaryotic cells are
|
A.
|
less complex than prokaryotic cells.
|
|
B.
|
members of the domains Bacteria and Archaea.
|
|
C.
|
defined by the presence of a membrane bound
nucleus.
|
|
D.
|
able to reproduce more rapidly than prokaryotes.
|
|
E.
|
less complex than prokaryotic cells, members of the
domains Bacteria and Archaea AND able to reproduce more rapidly than prokaryotes.
|
|
|
2.
|
The two magnifying lenses
found in a light microscope are the
|
|
3.
|
The resolving power of a
microscope is described as the ability of the microscope to
|
A.
|
separate clearly two objects that are very close
together.
|
|
C.
|
separate the colors of an organism’s internal
structure.
|
|
D.
|
see structures at various depths in a tissue.
|
|
|
4.
|
In viewing a microscopic
specimen, oil is used to
|
A.
|
increase the refraction.
|
|
B.
|
decrease the refraction.
|
|
C.
|
increase the reflection.
|
|
D.
|
increase the resolution.
|
|
E.
|
decrease the refraction AND increase the
resolution.
|
|
|
5.
|
The use of oil with certain
high power objective lenses increases
|
B.
|
the amount of light that enters the objective lens.
|
|
E.
|
All of the choices are correct.
|
|
|
6.
|
If everything else is
equal, the best way to observe more details in a microscopic specimen is to
|
B.
|
increase magnification.
|
|
|
7.
|
The microscope which allows
the specimen to appear 3-dimensional is the
|
A.
|
phase contrast microscope.
|
|
B.
|
interference microscope.
|
|
C.
|
fluorescence microscope.
|
|
D.
|
dark-field microscope.
|
|
|
8.
|
Which of the following
microscope types would be least useful in viewing unstained living cells?
|
|
9.
|
Electron microscopes differ
from light microscopes in that
|
A.
|
electrons replace light.
|
|
B.
|
electromagnets replace glass lenses.
|
|
D.
|
magnification is higher.
|
|
E.
|
All of the choices are correct.
|
|
|
10.
|
Specimens can be observed
at the atomic level using a(n)
|
A.
|
scanning electron microscope.
|
|
B.
|
transmission electron microscope.
|
|
C.
|
atomic force microscope.
|
|
D.
|
All of the choices are correct.
|
|
E.
|
None of the choices is correct.
|
|
|
11.
|
Individual atoms on the
surface of prepared samples can be observed by using the
|
A.
|
phase contrast microscope.
|
|
B.
|
scanning electron microscope.
|
|
C.
|
dark-field microscope.
|
|
D.
|
atomic force microscope.
|
|
|
12.
|
Basic dyes
|
A.
|
have negative charges.
|
|
B.
|
have positive charges.
|
|
C.
|
are electrically neutral.
|
|
D.
|
contain both positively and negatively charged
particles.
|
|
|
13.
|
Which of the following
stains is/are considered differential?
|
E.
|
acid-fast stain AND Gram stain.
|
|
|
14.
|
The Gram stain and the
endospore stain both use
|
E.
|
basic dyes AND safranin.
|
|
|
15.
|
The order of reagents in
the Gram stain reaction are
|
A.
|
safranin, alcohol, methylene blue, iodine.
|
|
B.
|
crystal violet, iodine, alcohol, safranin.
|
|
C.
|
methylene blue, alcohol, safranin.
|
|
D.
|
crystal violet, alcohol, iodine, safranin.
|
|
|
16.
|
Which may result in Gram-positive
bacteria appearing to be Gram-negative?
|
B.
|
decolorizing too short
|
|
E.
|
decolorizing too long AND using old cultures
|
|
|
17.
|
The major criteria used in placing
bacteria into different groups is based on differences in
|
B.
|
cell membrane permeability.
|
|
C.
|
presence or absence of flagella.
|
|
D.
|
detergent susceptibility.
|
|
|
18.
|
In a basic staining
procedure, which is the correct order?
|
C.
|
fix, stain, decolorize
|
|
D.
|
smear, decolorize, stain
|
|
|
19.
|
The acid-fast stain
|
A.
|
reflects differences in cytoplasmic membrane
structure.
|
|
B.
|
is useful for distinguishing a small group of
organisms, including Mycobacterium.
|
|
C.
|
uses crystal violet and safranin.
|
|
D.
|
uses carbolfuchsin and methylene blue.
|
|
E.
|
is useful for distinguishing a small group of
organisms, including Mycobacterium AND
uses carbolfuchsin and methylene blue.
|
|
|
20.
|
Capsules
|
B.
|
may correlate with an organism’s ability to cause
disease.
|
|
C.
|
are typically “negatively” stained.
|
|
D.
|
are stained as a wet mount.
|
|
E.
|
may correlate with an organism’s ability to cause
disease, are typically “negatively” stained AND are stained as a wet mount.
|
|
|
21.
|
The endospore stain
|
A.
|
is applicable to only a few groups of bacteria.
|
|
B.
|
usually shows the spores as green structures among
a background of pink cells.
|
|
C.
|
uses crystal violet as the primary stain.
|
|
D.
|
is an example of a negative stain.
|
|
E.
|
is applicable to only a few groups of bacteria AND
usually shows the spores as green structures among a background of pink
cells.
|
|
|
22.
|
Which fluorescent stain would
be useful for distinguishing between dead and living bacteria?
|
|
23.
|
Immunofluorescence
|
A.
|
uses fluorescently tagged molecules.
|
|
B.
|
makes use of the specificity in binding of antibodies.
|
|
C.
|
utilizes acridine orange.
|
|
D.
|
would require a special UV microscope.
|
|
E.
|
uses fluorescently tagged molecules, makes use of
the specificity in binding of antibodies AND would require a special UV
microscope.
|
|
|
24.
|
Which term(s) refer(s) to
bacterial morphology?
|
|
25.
|
Which is not true of the
cytoplasmic membrane?
|
A.
|
It defines the boundaries of the cell.
|
|
B.
|
It is a semipermeable barrier.
|
|
C.
|
It consists mainly of a fixed, static phospholipid
bilayer.
|
|
D.
|
It uses proteins as selective gates and sensors.
|
|
E.
|
All of the choices are true.
|
|
|
26.
|
Which is true of simple
diffusion of water?
|
A.
|
Water usually enters a cell and produces a
tremendous osmotic pressure.
|
|
B.
|
Water usually leaves the cell and produces negative
osmotic pressure.
|
|
C.
|
Water tends to enter and leave the cell equally,
resulting in no pressure in the cell.
|
|
D.
|
The diffusion ultimately relies on the selectively
permeable nature of the cell membrane.
|
|
E.
|
Water usually enters a cell and produces a
tremendous osmotic pressure AND the diffusion ultimately relies on the
selectively permeable nature of the cell membrane.
|
|
|
27.
|
The cytoplasmic membrane of
both eukaryotes and prokaryotes functions to
|
A.
|
form endoplasmic reticulum.
|
|
C.
|
regulate movement of molecules which enter and
leave the cell.
|
|
D.
|
form lysosomes and golgi apparatus.
|
|
|
28.
|
The proteins of bacteria
that are involved in the movement of small molecules into the cell, are
called
|
E.
|
transport proteins, permeases AND carriers.
|
|
|
29.
|
Most solutes pass through
the cytoplasmic membrane via
|
|
30.
|
Facilitated diffusion and
active transport
|
A.
|
both transport molecules into or out of a cell.
|
|
B.
|
are both not very specific as to which molecules
are transported.
|
|
C.
|
both require a concentration gradient to function.
|
|
D.
|
both require an expenditure of energy in order to
transport the molecules.
|
|
E.
|
both require a concentration gradient to function
AND both require an expenditure of energy in order to transport the
molecules.
|
|
|
31.
|
The macromolecule found in
the cell walls of all bacteria is
|
|
32.
|
Which is(are) true concerning
the cell wall of prokaryotes?
|
A.
|
It determines the shape of the bacteria.
|
|
B.
|
It prevents the bacteria from bursting.
|
|
C.
|
It contains peptidoglycan.
|
|
D.
|
It may be targeted by antimicrobials.
|
|
E.
|
All of the choices are true.
|
|
|
33.
|
Which amino acid(s) is/are
found only in the cell walls of bacteria?
|
E.
|
L-form of glycine AND L-form of methionine
|
|
|
34.
|
The cell wall of Gram-positive
bacteria
|
A.
|
contains a thin layer of peptidoglycan.
|
|
B.
|
contains a thick layer of peptidoglycan.
|
|
C.
|
is, due to its thickness, an excellent barrier to
most molecules.
|
|
D.
|
contains an outer membrane containing LPS.
|
|
E.
|
contains a thin layer of peptidoglycan AND contains
an outer membrane containing LPS.
|
|
|
35.
|
Which molecules are
associated with the cell wall of Gram-positive bacteria?
|
E.
|
peptidoglycan, D-form amino acids AND teichoic
acids
|
|
|
36.
|
The cell wall of
Gram-negative organisms
|
A.
|
has a thick peptidoglycan layer.
|
|
B.
|
has a thin peptidoglycan layer.
|
|
C.
|
is more permeable to various molecules than the Gram-positive
cell wall.
|
|
D.
|
is characterized by an outer membrane containing
LPS.
|
|
E.
|
has a thin peptidoglycan layer AND is characterized
by an outer membrane containing LPS.
|
|
|
37.
|
Endotoxin
|
B.
|
determines bacterial shape.
|
|
C.
|
may have different effects depending on the
specific bacterial source.
|
|
D.
|
is toxic due to the effects of the peptide side
chains.
|
|
E.
|
determines bacterial shape, may have different
effects depending on the specific bacterial source AND is toxic due to the
effects of the peptide side chains.
|
|
|
38.
|
Penicillin would be most
effective against
|
C.
|
Gram-positive bacteria.
|
|
D.
|
Gram-negative bacteria.
|
|
E.
|
growing bacteria AND Gram-positive bacteria.
|
|
|
39.
|
Peptidoglycan
|
A.
|
may be digested by penicillin.
|
|
B.
|
consists of a long string of NAG coupled to a long
string of NAM.
|
|
C.
|
may be digested by lysozyme.
|
|
D.
|
is used to construct a spheroplast.
|
|
|
40.
|
Which of the following
bacteria lack a cell wall?
|
B.
|
Mycobacterium
tuberculosis
|
|
|
41.
|
The capsule
|
A.
|
may be used for protection.
|
|
B.
|
may be used to help the bacteria adhere to surfaces.
|
|
C.
|
may be involved in movement.
|
|
D.
|
may be involved in energy production.
|
|
E.
|
may be used for protection AND may be used to help
the bacteria adhere to surfaces.
|
|
|
42.
|
The structures used for
motility in both eukaryotes and prokaryotes are
|
|
43.
|
Movement in bacteria
|
A.
|
is directly to or away from a stimulus.
|
|
B.
|
relies on the beating of cilia.
|
|
C.
|
is often referred to as run and tumble.
|
|
E.
|
is often referred to as run and tumble AND may
involve pili.
|
|
|
44.
|
Extrachromasomal DNA is
found in ____________________________.
|
E.
|
mitochondria AND plasmids
|
|
|
45.
|
Endospores are
|
B.
|
a form of reproduction.
|
|
C.
|
an obligate intracellular parasite.
|
|
D.
|
sensitive to damaging environmental conditions.
|
|
|
46.
|
Eukaryotic cells
|
A.
|
are more obviously compartmentalized than prokaryotes.
|
|
B.
|
usually have a single circular supercoiled piece of
DNA.
|
|
C.
|
contain peptidoglycan in the cell wall.
|
|
D.
|
have the same size ribosomes as prokaryotes.
|
|
E.
|
usually have a single circular supercoiled piece of
DNA AND contain peptidoglycan in the cell wall.
|
|
|
47.
|
The membranes of eukaryotes
and mycoplasma
|
A.
|
contain peptidoglycan.
|
|
B.
|
contain sterols for “strength”.
|
|
D.
|
are fixed static structures.
|
|
|
48.
|
Phagocytosis
|
A.
|
is the ingestion of particles and may be performed
by animal cells.
|
|
B.
|
is the ingestion of particles and may be performed
by bacteria.
|
|
C.
|
is the secretion of proteins.
|
|
D.
|
is the formation of a lysosome.
|
|
E.
|
is the ingestion of particles and may be performed
by bacteria AND is the formation of a lysosome.
|
|
|
49.
|
The cytoskeleton
|
A.
|
is a dynamic structure composed of microtubules,
microfilaments and intermediate filaments.
|
|
B.
|
is a static structure that gives a rigid shape to
the cell.
|
|
C.
|
consists of flagella and cilia that are
internalized.
|
|
D.
|
is not necessary for movement or reproduction.
|
|
E.
|
is a static structure that gives a rigid shape to
the cell, consists of flagella and cilia that are internalized AND is not
necessary for movement or reproduction.
|
|
|
50.
|
The nucleus
|
A.
|
is a double membrane sac containing DNA and is
found in eukaryotes.
|
|
B.
|
is a single phospholipid membrane sac containing
prokaryotic DNA.
|
|
C.
|
is a smaller structure contained within the eukaryotic
nucleolus.
|
|
D.
|
cannot transport molecules to the cytoplasm due to
the double membrane barrier.
|
|
|
51.
|
Which is not true of
mitochondria and chloroplasts?
|
A.
|
They are found in all organisms.
|
|
B.
|
They contain DNA and 70S ribosomes.
|
|
C.
|
They are capable of performing protein synthesis.
|
|
|
52.
|
An advantage of the smaller
size of prokaryotes, compared to eukaryotes, is
|
A.
|
high surface area relative to low cell volume.
|
|
B.
|
more rapid growth rates.
|
|
C.
|
compartmentalization of cellular processes in
membrane-bound organelles.
|
|
D.
|
predators, parasites, and competitors constantly
surround them.
|
|
E.
|
high surface area relative to low cell volume AND
more rapid growth rates.
|
|
True / False Questions
|
53.
|
Bacillus and Clostridium are
medically relevant groups of bacteria that characteristically stain
acid-fast.
True False
|
|
54.
|
LPS is found in the outer
membrane of Gram-positive bacteria.
True False
|
|
55.
|
Drugs that target prokaryotic
protein synthesis would have no effect on eukaryotic protein synthesis.
True False
|
|
56.
|
Penicillin affects the
synthesis of phospholipids, thereby producing weak membranes and lysis of the
bacteria.
True False
|
|
57.
|
Endospores are involved in
bacterial reproduction.
True False
|
|
58.
|
Lysosomes are bags of
digestive enzymes found in prokaryotic cells.
True False
|
|
59.
|
Smooth endoplasmic
reticulum is involved in protein synthesis.
True False
|
|
60.
|
Mitochondria and
chloroplasts are thought to have once been free living bacteria that invaded
another cell.
True False
|
|
61.
|
Cilia and flagella project
from the cell and are not covered by cytoplasmic membrane.
True False
|
|
62.
|
Prokaryotes may ingest
particles via phagocytosis.
True False
|
Multiple Choice Questions
|
63.
|
An electron microscope must
place the specimen in a vacuum within a sealed chamber. Why?
|
A.
|
Electrons are particles-there are also particles in
air. Without a vacuum, the electrons would strike and be scattered by
the atoms/particles within the air.
|
|
B.
|
Because electrons are highly radioactive, and the
chamber must be completely sealed to prevent them from escaping and
contaminating the lab area.
|
|
C.
|
This is the method for fixing the specimen to the
slide grid for an electron microscope. Without fixing the specimen by
vacuum, it would slide off and we wouldn’t be able to visualize it.
|
|
D.
|
All of the above are true.
|
|
|
64.
|
An electron microscope must
use electromagnet ‘lenses’ shaped like donuts to direct the electrons
onto the specimen. Why aren’t they solid, like the lenses in a light
microscope?
|
A.
|
A magnetic field cannot be applied across a
completely solid object-there must be an opening within the object for the
field to be applied through.
|
|
B.
|
The user has to be able to physically look through
the magnets to focus the beam of electrons onto the specimen, since the
metal of the magnets is opaque. Without a hole in the middle, the
user wouldn’t be able to see!
|
|
C.
|
The electrons would destroy the electromagnet
material unless there was a physical hole for them to travel through on
their way to the specimen.
|
|
D.
|
Electrons are particles, and particles cannot
travel through a solid item. The hole in the middle allows them to
travel from the source of the electrons to the specimen.
|
|
|
65.
|
Which microscope would be
the BEST selection for examination of a virus?
|
A.
|
Confocal scanning laser microscope
|
|
B.
|
Atomic force microscope
|
|
C.
|
Dark-field light microscope
|
|
D.
|
Scanning electron microscope
|
|
|
66.
|
You want to examine the
structure of the protein coat of a virus by microscopy. Which
microscope is your best choice, and why?
|
A.
|
The scanning electron microscope-it has excellent
resolution and magnification, much higher than a light microscope, and can
clearly visualize viruses.
|
|
B.
|
An atomic force microscope-this has the highest
resolution and magnification of the microscopes we discussed. Here,
we want to visualize a subcomponent of the virus particles, so we need the
best value for resolution and magnification we can possibly achieve.
|
|
C.
|
A fluorescent microscope-this will let us tag the
protein coat with colored dyes in order to visualize it using this light
microscope at 1000x.
|
|
D.
|
A bright-field light microscope-of course, we’ll
need to stain the viruses before we can visualize them against the bright
white background at 1000x total magnification.
|
|
|
67.
|
Your instructor wants you
to bring in an example of a biofilm to your lab. Which choice is the
best selection for bringing in an intact biofilm for further study?
|
A.
|
Disconnecting and bringing in the old, mildewed
showerhead from the showers in your dorm.
|
|
B.
|
Scraping the mold off of the shower curtain in your
dorm’s bathroom into a paper cup using a butter knife.
|
|
C.
|
Using a toothpick to scrape plaque off of your
teeth and smearing it onto a slide to bring in to lab.
|
|
D.
|
Wiping a sponge across a slimy boulder in a stream
in a nearby park and bringing it in to lab.
|
|
|
68.
|
A newly developed
antibiotic drug shows promise by inhibiting prokaryotic 70S ribosomes in
initial studies. However, when animal studies are begun, it’s noted
that it also inhibits growth of animal cells. Aren’t prokaryotic
ribosomes different from eukaryotic ribosomes? How can this be
happening?
|
A.
|
Perhaps the ribosomes aren’t as different as we
thought, so a drug can affect and impair both of them.
|
|
B.
|
While the proteins made in the cytosol of
eukaryotic cells are, indeed, produced from the 80S eukaryotic ribosome,
mitochondria and chloroplasts possess 70S ribosomes. This drug might
be impairing the activity of chloroplasts in animal cells.
|
|
C.
|
While the proteins made in the cytosol of
eukaryotic cells are, indeed, produced from the 80S eukaryotic ribosome,
mitochondria and chloroplasts possess 70S ribosomes. This drug might
be impairing the activity of mitochondria in animal cells.
|
|
D.
|
Perhaps the scientists accidently combined their
cultures of animal and bacterial cells-this might indicate an impairment in
growth in the culture. In reality, it’s still just impairing the
bacterial cells.
|
|
|
69.
|
A research laboratory is
investigating environmental factors that would inhibit the growth of Archaea. One
question they have is if adding the antibiotic penicillin would be effective
in controlling their growth. What do you think the outcome would be if
they tried this?
|
A.
|
The penicillin wouldn’t affect the Archaea because it prevents crosslinking of
peptidoglycan-Archaea don’t have this
compound in their cell walls.
|
|
B.
|
The penicillin will inhibit cell wall formation in
the Archaea, killing them.
|
|
C.
|
The penicillin will slow down the growth of
the Archaea by damaging the
cell wall, but they will still be able to grow somewhat.
|
|
D.
|
The penicillin will enhance the growth of the Archaea by providing a rich nutrient source.
|
|
|
70.
|
A research lab is trying to
produce new antibiotics. They come up with drugs that detach the
hydrophilic heads from the phospholipids in plasma membranes. This drug
|
A.
|
would be highly toxic and completely useless for
human beings-we also have plasma membranes with hydrophilic head groups!
|
|
B.
|
would be highly toxic only to bacteria, making it
an excellent drug to develop and test further. Humans have a
different phospholipid structure in their cell plasma membranes than
bacteria.
|
|
C.
|
would generally not be toxic enough to damage Gram-negative
bacteria, as their plasma membranes lie under a thick layer of
peptidoglycan.
|
|
D.
|
would be highly toxic for human beings, but might
be useful as a topical antibiotic skin cream. The top layer of skin
cells is dead anyway, so it wouldn’t matter if this damaged those cells.
It could NOT be taken internally, though, by human beings.
|
|
|
71.
|
A new drug is developed
that targets and binds to the lipid A portion of LPS from Gram-negative
bacterial cells. This drug shows a high degree of activity and binding
in a test tube setting against purified lipid A. Based on this
information,
|
A.
|
we should fast track this drug and get it out to
physicians immediately to help prevent toxic/septic shock in humans.
|
|
B.
|
we should do some animal testing with whole
Gram-negative cells and the drug before we jump to any conclusions.
|
|
C.
|
we should next proceed by moving to testing in a
test tube using whole Gram-negative cells to see if it binds with the same
strength.
|
|
D.
|
we should abandon the drug entirely. Binding
to lipid A won’t kill the bacteria, so the drug is useless to develop
further. It wouldn’t help humans who have septic shock at all.
|
|
|
72.
|
Another new drug is
developed-this one inhibits formation of the flagellar filament by impairing
production of the protein flagellin. Is this going to be a good drug?
|
A.
|
Potentially-it would definitely only impair
bacteria, but wouldn’t matter for the ones that don’t use a flagellum to
move around.
|
|
B.
|
Potentially-if it could be shown that it also
didn’t impair the production of the eukaryotic flagellum.
|
|
C.
|
No-our eukaryotic cells depend on flagella to move
around in our body, and this would impair that function.
|
|
D.
|
No-bacterial flagella are produced using
microtubules, not the protein flagellin. This drug would be useless
in eliminating bacteria.
|
|
Ch03 Key
Multiple Choice Questions
|
1.
|
Eukaryotic cells are
|
A.
|
less complex than prokaryotic cells.
|
|
B.
|
members of the domains Bacteria and Archaea.
|
|
C.
|
defined by the presence of a membrane bound
nucleus.
|
|
D.
|
able to reproduce more rapidly than prokaryotes.
|
|
E.
|
less complex than prokaryotic cells, members of the
domains Bacteria and Archaea AND able to reproduce more rapidly than
prokaryotes.
|
|
|
Bloom’s Level: 2.
Understand
Learning Outcome: 03.01
Section: 03.01
Topic: Eukaryotes
|
|
2.
|
The two magnifying lenses
found in a light microscope are the
|
|
Bloom’s Level: 1. Remember
Learning Outcome: 03.01
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
3.
|
The resolving power of a
microscope is described as the ability of the microscope to
|
A.
|
separate clearly two objects that are very close
together.
|
|
C.
|
separate the colors of an organism’s internal
structure.
|
|
D.
|
see structures at various depths in a tissue.
|
|
|
Bloom’s Level: 2.
Understand
Learning Outcome: 03.01
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
4.
|
In viewing a microscopic
specimen, oil is used to
|
A.
|
increase the refraction.
|
|
B.
|
decrease the refraction.
|
|
C.
|
increase the reflection.
|
|
D.
|
increase the resolution.
|
|
E.
|
decrease the refraction AND increase the resolution.
|
|
|
Bloom’s Level: 1. Remember
Learning Outcome: 03.01
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
5.
|
The use of oil with certain
high power objective lenses increases
|
B.
|
the amount of light that enters the objective lens.
|
|
E.
|
All of the choices are correct.
|
|
|
Bloom’s Level: 2.
Understand
Learning Outcome: 03.01
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
6.
|
If everything else is
equal, the best way to observe more details in a microscopic specimen is to
|
B.
|
increase magnification.
|
|
|
Bloom’s Level: 3. Apply
Learning Outcome: 03.01
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
7.
|
The microscope which allows
the specimen to appear 3-dimensional is the
|
A.
|
phase contrast microscope.
|
|
B.
|
interference microscope.
|
|
C.
|
fluorescence microscope.
|
|
D.
|
dark-field microscope.
|
|
|
Bloom’s Level: 1. Remember
Learning Outcome: 03.02
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
8.
|
Which of the following
microscope types would be least useful in viewing unstained living cells?
|
|
Bloom’s Level: 3. Apply
Learning Outcome: 03.02
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
9.
|
Electron microscopes differ
from light microscopes in that
|
A.
|
electrons replace light.
|
|
B.
|
electromagnets replace glass lenses.
|
|
D.
|
magnification is higher.
|
|
E.
|
All of the choices are correct.
|
|
|
Bloom’s Level: 1. Remember
Learning Outcome: 03.02
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
10.
|
Specimens can be observed
at the atomic level using a(n)
|
A.
|
scanning electron microscope.
|
|
B.
|
transmission electron microscope.
|
|
C.
|
atomic force microscope.
|
|
D.
|
All of the choices are correct.
|
|
E.
|
None of the choices is correct.
|
|
|
Bloom’s Level: 1. Remember
Learning Outcome: 03.02
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
11.
|
Individual atoms on the
surface of prepared samples can be observed by using the
|
A.
|
phase contrast microscope.
|
|
B.
|
scanning electron microscope.
|
|
C.
|
dark-field microscope.
|
|
D.
|
atomic force microscope.
|
|
|
Bloom’s Level: 2.
Understand
Learning Outcome: 03.02
Section: 03.01
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
12.
|
Basic dyes
|
A.
|
have negative charges.
|
|
B.
|
have positive charges.
|
|
C.
|
are electrically neutral.
|
|
D.
|
contain both positively and negatively charged
particles.
|
|
|
Bloom’s Level: 1. Remember
Learning Outcome: 03.03
Section: 03.02
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
13.
|
Which of the following
stains is/are considered differential?
|
E.
|
acid-fast stain AND Gram stain.
|
|
|
Bloom’s Level: 1. Remember
Learning Outcome: 03.03
Section: 03.02
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
|
14.
|
The Gram stain and the
endospore stain both use
|
E.
|
basic dyes AND safranin.
|
|
|
Bloom’s Level: 1. Remember
Learning Outcome: 03.03
Section: 03.02
Topic: Tools and Methods
of Culturing, Classifying, and Identify Microorganisms
|
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